When comparing alternative mapping strategies correcting for known variances and including multiple genome assemblies to the commonly used method (S13 Fig in S4 File), some genes (HLA-DQA1, HLA-DRB1) can change direction of association to CF lung disease from imputed gene expression, even though overall disease association are correlated (Fig 7) among the commonly imputable genes, as described [81, 82]. The gene discussed is HLA-DRB1; the disease is cystic fibrosis.