Furthermore, when ethanol-dissolved oxysterols, 27- and 24-OHC were delivered at high concentration (8-fold higher than physiological plasma concentrations) to HCC cancer cells lines, HepG2 and Huh7, total oxysterols and cholesterol in secreted lipoproteins were significantly increased implying that ACAT2 mediated oxysterol secretion may be protect HCC tumors from excess oxysterol/cholesterol accumulation (Lu et al., 2013). The gene discussed is ACAT2; the disease is hepatocellular carcinoma.