DYSF and neuromuscular disease caused by qualitative or quantitative defects of dysferlin: Dysferlinopathy-specific features: Absent Dysferlin stain in muscle immunohistochemistry (IHC), and disease-range blood monocyte assay for DYSF protein estimation; Difficulty running, change in gait, wasting of calves, biceps lump; Most-affected muscles: Gastrocnemius, Iliopsoas, hip adductors, hamstrings and quadriceps