Silencing ANO1 had no effect on AKT phosphorylation induced by EGF in human pancreatic cancer cells (Crottes et al., 2019), or in urinary bladder carcinoma T24 cells and squamous cell carcinoma SCC-1 cells (Duvvuri et al., 2012) under normal cell culture growth conditions. This evidence concerns the gene ANO1 and familial pancreatic carcinoma.