Compared to sorafenib-treated si-NC HCC cells, knockout of FOXO3a obviously upregulated expression of p62 and downregulated expression of LC-3, which meant the activation of autophagic flux induced by sorafenib was blocked by knockout of FOXO3a in 4 HCC cells (Fig. 4B). This evidence concerns the gene MAP1LC3A and hepatocellular carcinoma.