To confirmed the role of miR-146a together with its negatively regulated vimentin in the tumorigenesis of ESCC cells, the CE81FN+ + CON stable cell line shown in Fig. 3 was transiently transfected with scramble microRNA or mimic-miR-146a, and miR-146a overexpression was confirmed by real-time PCR (Fig. 4a). The gene discussed is VIM; the disease is esophageal squamous cell carcinoma.