VIM and esophageal squamous cell carcinoma: In addition, we transiently transfected two ESCC cell lines KYSE 150 (high miR-146a expression) and KYSE 70 (low miR-146a expression) (Additional file 1: Figure S3A) with anti-miR-146a or miR-146a, respectively, and evaluated the level of vimentin protein (Additional file 1: Figure S3B and S3C) and cell invasion capability (Additional file 1: Figure S3D).