Moreover, considering that EYS is the major causative gene of RP in Japan8,12,23–26, we constructed the stepwise genetic screenings of patients with RP—starting with the direct Sanger sequencing for the two EYS founder mutations in Japan, followed by, targeted NGS for all exons of the EYS gene, and whole genome sequencing (WGS). The gene discussed is EYS; the disease is retinitis pigmentosa 1.