We detected a significant increase in both CD4 and CD8 frequencies of the total live cell content when compared the PC cohort to healthy donor samples (4.3% vs 1.3%, p = 0.0007 and 3.6% vs 1.4%, p = 0.0037, PC vs HBM, respectively), however, the fold increase was higher in the CD4 subset than in CD8 (3.4 vs 2.6-fold, respectively) suggesting a robust CD4 infiltration in PC patients accounting for the skewed CD4 to CD8 ratio. This evidence concerns the gene CD8A and pachyonychia congenita.