Our results from RNA-seq with the in vitro and in vivo findings suggested that CBD might induce the expression of DUSP1 and KLF6, following the HNSCC tumor suppression via the DUSP1 and/or KLF6 dependent activation of apoptosis and autophagy cellular signaling on HNSCC. Here, KLF6 is linked to head and neck squamous cell carcinoma.