We also verified that miR-515-3p-overexpressing cells co-overexpressing vimentin and MMP3 showed higher invasive and migration ability as compared with the miR-515-3p-overexpressing cells overexpressing vimentin or MMP3 alone, and almost completely restored the suppressive effect of miR-515-3p on ESCC invasive and migration. This evidence concerns the gene VIM and esophageal squamous cell carcinoma.