On the contrary, the expression of vimentin and MMP3 in miR-515-3p-knockdwon ESCC cell lines (KYSE150-Luc-ZIP-miR-515-3p, EC109-ZIP-miR-515-3p) was further inhibited with shRNA (Fig. 5c). This evidence concerns the gene MMP3 and esophageal squamous cell carcinoma.