The following variables proved most important in prediction when comparing QFS with HC; 4E-BP1, CD40, AXIN1, CCL11, CD244, IL-8, OPG, CCL4, TRAIL, and CD8A, CFS with HC; 4E-BP1, CDCP1, AXIN1, MMP-10, CSF-1, TNFB, NT-3, FGF-23, IL-12B, and IL-8, and QFS and CFS with HC; 4E-BP1, AXIN1, CD40, CDCP1, CSF-1, IL-8, FGF-23, CCL4, ADA, and MMP-10 (Additional file 3: Figure S3). The gene discussed is CD40; the disease is myalgic encephalomeyelitis/chronic fatigue syndrome.