The expression of Vimentin in MCF-7 and MDA-MB-231 breast cancer cells co-cultured with CD133+ HPCs (1.5 ± 0.19,1.3 ± 0.11 respectively) was significantly higher than that of co-cultured with CD133- HPCs (0.7 ± 0.11, 0.5 ± 0.13 respectively) and single breast cancer cells (0.6 ± 0.09, 0.41 ± 0.16 respectively; P < 0.05). The gene discussed is PROM1; the disease is breast cancer.