GPC3 and hepatocellular carcinoma: To understand activation of the classical Wnt/β-Catenin signal in HCC cells, we first detected the expression of Wnt ligand and receptor crimp protein (frizzled, FZD or Frz) in various HCC cell lines: HepG2 (GPC3++), Huh-7 (GPC+), and Li-7 (GPC3−).