These results are in contrast, to a previous report, showing that chronic hyperglycemia induces glucagon protein expression in fully differentiated mouse beta-cells (Brereton et al., 2014), thus, proposing that the immature status of the bi-hormonal INS-1 cells prohibit the ability to study proglucagon-derived peptides in response to glucose stimulation. This evidence concerns the gene GCG and Hyperglycemia.