The inhibition of Snail, Slug or Twist through siRNA or antisense transfer leads to inhibition of tumor metastasis or growth inhibition and increased susceptibility to chemotherapeutic agents (Vannini et al., 2007; Zhang et al., 2008, 2010), which demonstrates the potential of manipulating EMT markers to achieve a better outcome in bladder cancer treatment. Here, TWIST1 is linked to urinary bladder cancer.