Meanwhile, the addition of AKT inhibitor blocked the radiation-induced Treg cell survival in bladder cancer cell, which can inhibit CD8+ T cells in the tumor tissue to compromise the antitumor activities (43). We found that the effect of ADNP on tumor proliferation was rescued by AKT inhibitors, suggesting that ADNP promotes cell proliferation mainly through activation of the AKT pathway in BC. This evidence concerns the gene AKT1 and urinary bladder cancer.