CTCs were defined as nucleated cells expressing cytokeratin and lacking CD45 (Figure 3f).[29] Using this CTC capture film, both single individual CTCs and CTC clusters (a group of more than two tumor cells) were successfully isolated 2–499 CTCs from 2 mL blood samples taken from 11 patients with different kinds of advanced cancer (Figure 3d; Table S3, Supporting Information), including lung cancer, nasopharyngeal cancer, laryngeal cancer, nasopharyngeal cancer, ovarian cancer, breast cancer, and colorectal cancer with a high detection rate of 100% (Figure 3g). The gene discussed is PTPRC; the disease is nasopharyngeal carcinoma.