Study researched the number of Melan-A/Mart-1-specific CD8 T cells in patients after isolated limb perfusion with hyperthermia and found a small increase in tumor-specific T-cells in a subpopulation of patients with melanoma, demonstrating the potential of thermal therapy in the activation and differentiation of immune effector cells in the tumor microenvironment (97). This evidence concerns the gene MLANA and melanoma.