Genes involved in the biosynthesis of asparagine (Asns) and serine (Phgdh) and in folate metabolism (Mthfd1l and Mthfd2) were also elevated in Nod2−/− DMBA + HFD mice and are associated with the development of hepatocellular carcinoma. The gene discussed is MTHFD2; the disease is hepatocellular carcinoma.