We designed a 5′ biotin-labeled TERT promoter (−387 to −137) probe to pull down the TERT promoter binding proteins by biotin–streptavidin-beads pull-down (BSBP) method and identified NCOA3 as a novel TERT promoter binding protein binds to the −234 to −144 region of the TERT promoter in HCC cells. This evidence concerns the gene NCOA3 and hepatocellular carcinoma.