Figure 6D summarizes the expression and distribution of PGC1α in neurons and astrocytes in all analyzed conditions (ctrl vs ctrl, p = 0.0001; AD vs AD, p = 0.0001; NDAN vs NDAN, p = 0.0028). Wb analyses conducted on total protein extracts confirmed the significant downregulation of PGC1α in AD frontal cortex compared with control subjects and NDAN individuals, with the latter displaying levels similar to those of control subjects (Extended Data Fig. 6-1). This evidence concerns the gene PPARGC1A and Alzheimer disease.