However, our study is limited to the fact that we did not investigate the level of parasitaemia and other haemoglobinopathies such as Glucose-6-phosphate dehydrogenase (G6PD), and alpha thalassaemia which is known to have epistasis effects on sickle cell gene with respect to P. falciparum malaria [65]. This evidence concerns the gene G6PD and hemoglobinopathy.