HC-HMGB1−/− mice developed severe steatosis as early as 2 weeks after HFD, and this was maintained for up to 16 weeks (Fig. 3a).WT mice showed evidence of fat accumulation in liver after 16 weeks of HFD, but this was less extensive than in HC-HMGB1−/− mice (Fig. 3a). This evidence concerns the gene HMGB1 and steatosis.