For example, the direct binding of miR-525-5p to MAGI2-AS3 and negative regulation of miR-525-5p by MAGI2-AS3 in human ovarian cancer cell lines was proven using transfection by plasmids, qRT-PCR, dual-luciferase reporter assay, pull-down assay, and RIP with anti-AGO2 vs anti-IgG antibodies [69]. The gene discussed is PDS5B; the disease is ovarian cancer.