We applied three different panels to demonstrate the levels of VISTA in tumor-infiltrating CD68+ macrophages, CD3+ T cells, CD19+ B cells, CD4+ T-helper cells, and CD8+ cytotoxic T cells, as well as to explore the association between VISTA expression and PD-1/PD-L1 (Supplementary Table S1 and Fig S2). This evidence concerns the gene CD4 and neoplasm.