On the other hand, is that means TWIST1 involved in another steps of the tumor invasion and metastasis process other than the TWIST1/E-cadherin/EMT pathway[23] which was confirmed in Satorus[12] and Xies[16] research, such as the formation of invadopodia,[13] intravascular migration, extravasation[24] and vasculogenic mimicry formation,[25] these pathologic functions were demonstrated in vitro cell experiments, testing proteins, such as VEGFα, ITGβ1, and VE-cadherin in our samples may help us further understand TWIST1 function in the progression of EC. This evidence concerns the gene CDH5 and neoplasm.