To validate the specific effect of LINC01140 on the aggressiveness of bladder carcinoma cells and the development of MIBC, we transfected si-LINC01140 to generate LINC01140 knockdown in T24 cells, and performed real-time PCR to verify the transfection efficiency (Figure 4A). This evidence concerns the gene LINC01140 and urinary bladder carcinoma.