Furthermore, double staining for macrophage marker—Mac-2 and MPO (Fig. 3A), and MPO oxidation products—3-chlorotyrosine (Fig. 3B), and 3-nitrotyrosine (Fig. 3C) revealed decreased colocalization of MPO and oxidation markers in the macrophages in lesions from CKD-bMPOKO mice as compared with CKD-bMPOWT mice. This evidence concerns the gene MPO and chronic kidney disease.