On the contrary, the five remaining colon cancer cell lines, namely SW48, DIFI, MICOL24, CACO-2 and E705, showed no hyperactivating mutations in KRAS, NRAS, BRAF and PIK3CA genes, with the E705 cell line carrying a silent mutation in PIK3CA gene. The gene discussed is KRAS; the disease is malignant colon neoplasm.