Analysis of VRK1 gene promoter methylation status in an NB tumor panel shows a region, downstream of the VRK1 transcription start site, that is hypomethylated in high-risk tumors with MYCN amplification and is defined by a single CpG probe (cg26685539, mean methylation difference 0.2 (b), q < 0.001)) (Figure 6a, Table S5). Here, MYCN is linked to neuroblastoma.