In the same tumour cohort, complete loss of RNF20 assessed using immunohistochemical staining was seen in only 6% of tumours (26 of 424) and did not correlate with loss of H2Bub1, implying that abrogation of RNF20 function does not explain the majority of H2Bub1 loss present in these tumours. This evidence concerns the gene RNF20 and neoplasm.