However, the pI value of SM deacylase was different from our previous report using analytical IEF of a homogenate of the SC of AD skin, which detected pI values of SM deacylase, GlcCDase, aSMase and aCDase of 4.2, 7.4, 7.0 and 5.7, respectively [19]. This evidence concerns the gene ASAH1 and Alzheimer disease.