The limit of detection (LoD) of the multiplex ddPCR assay, used in this study, was determined to be equal to 0.2%, which is in agreement with the reported assay sensitivity, demonstrating its applicability for rapid, robust and highly sensitive quantification of KRAS G12/G13 mutations in NSCLC patients’ plasma samples. This evidence concerns the gene KRAS and non-small cell lung carcinoma.