The mRNA expression of anabolic factors such as aggrecan, collagen type I and type X, tissue inhibitor of metalloprotease (TIMP)-1, and TIMP-3 was significantly increased in rats in FJH-KO 150 group compared with that in rats with MIA induced osteoarthritis, but the expression of collagen type II mRNA did not differ significantly among all the MIA-treated groups. The gene discussed is TIMP1; the disease is osteoarthritis.