NOS2 and central nervous system cancer: Whereas ZIKV treatment of CT2A glioma also led to rapid increases in the numbers of Ly6C+ monocytes (~16.5-fold) and inducible NOS–producing Ly6C+ monocytes (~24-fold) in the brain by day 14, it was not associated with activation of microglia, F4/80+ macrophages, or CD11b+ monocyte-derived DCs (Figure 2B).