To further testify the relationship between miR-18b-5p and FBP1 in ESCC cells, Eca109 cells were co-transfected with reporter plasmid contained wild type (wt) or mutant type (mut) of 3’UTR of FBP1 and miR-18b-5p mimic. This evidence concerns the gene FBP1 and esophageal squamous cell carcinoma.