The results showed that HNSCC samples in the high-risk group contained a lower fraction of naïve B cells (P < 0.05), CD8 T cells (P < 0.001), CD4 memory activated T cells (P < 0.001) and follicular helper T cells (P < 0.001) compared to those in the low-risk group. This evidence concerns the gene CD4 and head and neck squamous cell carcinoma.