In this study, ZnO-NPs were used as drug carriers to carry both Cp and Gem (ZnO-NPs(Cp/Gem)), and then the effects of ZnO-NPs(Cp/Gem) on cell sensitivity, apoptosis and mitochondrial function were investigated in lung cancer A549 cells, which may contribute to understanding the effect and application value of ZnO-NPs(Cp/Gem) in lung cancer chemotherapy. The gene discussed is GEM; the disease is lung cancer.