This study revealed that ZnO-NPs(Cp), ZnO-NPs(Gem), and ZnO NPs(Cp/Gem) exerted the better anticancer effect, especially ZnO NPs(Cp/Gem), in compared with free Cp, Gem and Cp+Gem, which suggested that ZnO-NPs improved the long-term chemotherapy efficacy for cancer cells. This evidence concerns the gene GEM and cancer.