The result of CCK8 assay showed that the OD value was significantly decreased after depletion of ARHGEF39 in Caki-1, 786-O and UT33A cells (p < 0.01, Figure 3D-F), indicating that depletion of ARHGEF39 suppressed the viability of ccRCC cells. The gene discussed is ARHGEF39; the disease is nonpapillary renal cell carcinoma.