Tnfsf14 absence apparently alleviated UUO-induced tubular injury and renal fibrosis, as demonstrated by significant reductions in collagen deposition by staining with Sirius Red and Masson’s trichrome (Figure 3A), in the expression of α-SMA and fibronectin by immunohistochemical staining and western blot (Figure 3B and 3C), in the mRNA levels of pro-fibrotic markers Cola1,Vim, and TGF-β1 by qRT-PCR (Figure 3D), and in tubular dilation, brush border disruption and tubular atrophy (Supplementary Figure 4A and 4B). This evidence concerns the gene ACTA1 and renal fibrosis.