SPAG5 and triple-negative breast carcinoma: Second, we depleted (using siRNAs) the expression of three main effectors of the pathway, YAP, TAZ, and TEAD, in MCF-10A cells stably overexpressing SPAG5, and in three different triple-negative breast cancer cell lines, MDA-MB-231, MDA-MB-468, and SUM-159PT.