As expected, the stem–loop sequences in the 3’UTRs of CCNE1 and MCM2 could be amplified in the GFP antibody pulldown group but not in the group using isotype IgG (Fig. 6h), indicating the binding of Roquin1 to the 3’UTRs of cell cycle–promoting mRNAs in breast tumor cells. This evidence concerns the gene MCM2 and breast neoplasm.