AGPAT2, which induces the expression of PPARγ and C/EBPα [45], as well as critical genes governing adipocyte function such as ADIPOQ, PLIN1, SLC2A4, FABP4 and LPL were also up-regulated in PCOS cells with peak expression level at days 7–12 following peak chromatin accessibility generally at day 3 (Fig. 2, Additional file: Supplemental Fig. 7), suggesting chromatin opening and TF binding to precede and potentially influence transcription at these genes. The gene discussed is ADIPOQ; the disease is polycystic ovary syndrome.