We screened IFN-γ production in response to peptide pools containing 15mer overlapping peptides from: (1) Cytomegalovirus (CMV) matrix protein 65 (pp65); (2) Candida albicans mannoprotein MP65; (3) a mixture of 14 previously identified optimal MHC class-II restricted epitopes from human cytomegalovirus (HHV-5), Epstein-Barr virus (HHV-4), influenza A, and Clostridium tetani toxoid (pool named CEFT); (4) HIV-1 Gag; (5) HIV-1 Pol; (6) HIV-1 Env; and (7) HIV-1 Nef. Here, IFNG is linked to influenza.