On the 18th day, when the difference in the protective effect between N-exo- and G-exo-stimulated DC vaccination was apparent, we evaluated the generation of tumor-reactive multifunctional T cells that can concurrently express multiple Th1 cytokines (including IFN-γ, TNF-α, and IL-2) using multicolor intracellular cytokine staining and flow cytometry (Figure 4C). This evidence concerns the gene TNF and neoplasm.