Remarkably, despite the rather high rate of lung infection clearance, all aP-immunized mice—irrespective of the addition of FIM2/3 with LOS and/or dACT, and despite the presence of 0.4 μg of PRN per vaccine dose and of ACT-neutralizing antibodies (Figure S2b)—again maintained a steady level of B. pertussis colonization at 105 CFU per nose for 50 days post infection (Figure 3b). This evidence concerns the gene DHCR7-DT and infection.