EGF and colorectal cancer: Full-length N-term-out functional EGFR ligands Amphiregulin (AREG), heparin binding EGF (HB-EGF) and transforming growth factor alpha (TGF)-α were first identified in sEVs derived from a panel of breast and colorectal cancer cell lines using fluorescence-activated vesicle sorting (FAVS) [120].