In addition, in contrast to the effects observed in established colon carcinoma cell lines, 1,25(OH)2D3 does not affect the expression of key genes of the Wnt/β-catenin pathway (AXIN2, CCND1, DKK1 and c-MYC) in human colon tumor or normal organoids derived from CRC patients, where only the DKK4 Wnt/β-catenin target gene is repressed by 1,25(OH)2D3 [80]. Here, DKK4 is linked to colorectal carcinoma.