DNA from NCI-H1299 cells stably over-expressing RASSF1C, cells with RASSF1C knocked down, cells with PIWIL1 knocked down, or cells expressing sh-RNA vector control were used to perform global DNA methylation analyses using the RRBS method [14] to identify DMRs as candidates for new lung cancer biomarkers. Here, PIWIL1 is linked to lung cancer.