In line with our observations at the protein level by IHC (Figure 2), HIC1 expression was not detected in the three transformed epithelial cell lines LNCaP, PC3 and DU145, in close agreement with the silencing of HIC1 through promoter hypermethylation in prostate tumors [12–16] (Figure 4A). Here, HIC1 is linked to prostate neoplasm.